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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
15/12/2017 |
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Data da última atualização: |
15/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARKA, G. D.; CAIXETA, E. T.; ALMEIDA, R. F. de; ALVARENGA, S. M.; ZAMBOLIM, L. |
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Afiliação: |
Geleta Dugassa Barka, BIOCAFÉ/BIOAGRO/UFV; EVELINE TEIXEIRA CAIXETA, SAPC; Robson Ferreira de Almeida, Instituto Federal do Espírito Santo; Samuel Mazzinghy Alvarenga, FEPAGRO; Laércio Zambolim, Departamento de Fitopatologia/Universidade Federal de Viçosa. |
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Título: |
Differential expression of molecular rust resistance components have distinctive profiles in Coffea arabica - Hemileia vastatrix interactions. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
European Journal of Plant Pathology, v. 149, n. 3, p. 543-561, Nov. 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Countering the economic hurdle caused by coffee leaf rust disease is most appealing at this time as it has posed a major threat to coffee production around the world. Establishing differential expression profiles at different times following pathogen invasion in both innate and acquired immunities unlocks the molecular components of resistance and susceptibility. Suppression subtractive hybridization (SSH) was used to identify genes differentially over-expressed and repressed during incompatible and compatible interactions between Coffea arabica and Hemileia vastatrix. From 433 clones of expressed sequence tags (ESTs) sequenced, 352 were annotated and categorized of which the proportion of genes expressed during compatible interaction were relatively smaller. The result showed upregulation and downregulation of various genes at 12 and 24 h after pathogen inoculation in both interactions. The use of four different databases in searching for gene homology resulted in different number of similar sequences. BLASTx against EMBL-EBI (European Molecular Biology Laboratory-European Bioinformatics Institute) database being with the maximum (100%) hits for all the annotated sequences. RT-qPCR analysis of seven resistance-signaling genes showed similar expression patterns for most of the genes in both interactions, indicating these genes are involved in basal (nonspecific) defense during which immune reactions are similar. Using SSH, we identified different types of resistance related genes that could be used for further studies towards resistant cultivar development. The potential role of some of the resistance related proteins found were also discussed. MenosCountering the economic hurdle caused by coffee leaf rust disease is most appealing at this time as it has posed a major threat to coffee production around the world. Establishing differential expression profiles at different times following pathogen invasion in both innate and acquired immunities unlocks the molecular components of resistance and susceptibility. Suppression subtractive hybridization (SSH) was used to identify genes differentially over-expressed and repressed during incompatible and compatible interactions between Coffea arabica and Hemileia vastatrix. From 433 clones of expressed sequence tags (ESTs) sequenced, 352 were annotated and categorized of which the proportion of genes expressed during compatible interaction were relatively smaller. The result showed upregulation and downregulation of various genes at 12 and 24 h after pathogen inoculation in both interactions. The use of four different databases in searching for gene homology resulted in different number of similar sequences. BLASTx against EMBL-EBI (European Molecular Biology Laboratory-European Bioinformatics Institute) database being with the maximum (100%) hits for all the annotated sequences. RT-qPCR analysis of seven resistance-signaling genes showed similar expression patterns for most of the genes in both interactions, indicating these genes are involved in basal (nonspecific) defense during which immune reactions are similar. Using SSH, we identified different types of resistance related ... Mostrar Tudo |
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Palavras-Chave: |
Coffee; Plant-pathogen interaction; Rust disease. |
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Thesagro: |
Café; Ferrugem; Patogeno. |
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Thesaurus Nal: |
Gene expression; pest resistance. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169104/1/Differential-expression-of-molecular-rust-resistance.pdf
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Marc: |
LEADER 02491naa a2200265 a 4500
001 2082676
005 2017-12-15
008 2017 bl uuuu u00u1 u #d
100 1 $aBARKA, G. D.
245 $aDifferential expression of molecular rust resistance components have distinctive profiles in Coffea arabica - Hemileia vastatrix interactions.$h[electronic resource]
260 $c2017
520 $aCountering the economic hurdle caused by coffee leaf rust disease is most appealing at this time as it has posed a major threat to coffee production around the world. Establishing differential expression profiles at different times following pathogen invasion in both innate and acquired immunities unlocks the molecular components of resistance and susceptibility. Suppression subtractive hybridization (SSH) was used to identify genes differentially over-expressed and repressed during incompatible and compatible interactions between Coffea arabica and Hemileia vastatrix. From 433 clones of expressed sequence tags (ESTs) sequenced, 352 were annotated and categorized of which the proportion of genes expressed during compatible interaction were relatively smaller. The result showed upregulation and downregulation of various genes at 12 and 24 h after pathogen inoculation in both interactions. The use of four different databases in searching for gene homology resulted in different number of similar sequences. BLASTx against EMBL-EBI (European Molecular Biology Laboratory-European Bioinformatics Institute) database being with the maximum (100%) hits for all the annotated sequences. RT-qPCR analysis of seven resistance-signaling genes showed similar expression patterns for most of the genes in both interactions, indicating these genes are involved in basal (nonspecific) defense during which immune reactions are similar. Using SSH, we identified different types of resistance related genes that could be used for further studies towards resistant cultivar development. The potential role of some of the resistance related proteins found were also discussed.
650 $aGene expression
650 $apest resistance
650 $aCafé
650 $aFerrugem
650 $aPatogeno
653 $aCoffee
653 $aPlant-pathogen interaction
653 $aRust disease
700 1 $aCAIXETA, E. T.
700 1 $aALMEIDA, R. F. de
700 1 $aALVARENGA, S. M.
700 1 $aZAMBOLIM, L.
773 $tEuropean Journal of Plant Pathology$gv. 149, n. 3, p. 543-561, Nov. 2017.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
27/04/2010 |
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Data da última atualização: |
16/12/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MAR, T. B.; LAU, D.; NHANI JUNIOR, A.; SCHONS, J.; YAMAZAKI-LAU, E.; PEREIRA, J. F. |
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Afiliação: |
Talita Bernardon Mar, Estudante; DOUGLAS LAU, CNPT; ANTONIO NHANI JUNIOR, CNPT; JUREMA SCHONS, UPF; ELENE YAMAZAKI LAU, CNPT; JORGE FERNANDO PEREIRA, CNPT. |
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Título: |
Barley and cereal yellow dwarf virus genetic diversity in Brazil. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Virus: reviews and research, v. 14, p. 77-78, 2009. |
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Idioma: |
Inglês |
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Notas: |
Suplemento, ref. 060. Edição dos Resumos do XX National Meeting of Virology, Brasília, DF, nov. 2009. |
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Palavras-Chave: |
Disease. |
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Thesagro: |
Cevada. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/127714/1/SP-15759.pdf
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Marc: |
LEADER 00621nam a2200193 a 4500
001 1852842
005 2019-12-16
008 2009 bl uuuu u00u1 u #d
100 1 $aMAR, T. B.
245 $aBarley and cereal yellow dwarf virus genetic diversity in Brazil.
260 $aVirus: reviews and research, v. 14, p. 77-78, 2009.$c2009
500 $aSuplemento, ref. 060. Edição dos Resumos do XX National Meeting of Virology, Brasília, DF, nov. 2009.
650 $aCevada
653 $aDisease
700 1 $aLAU, D.
700 1 $aNHANI JUNIOR, A.
700 1 $aSCHONS, J.
700 1 $aYAMAZAKI-LAU, E.
700 1 $aPEREIRA, J. F.
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Embrapa Trigo (CNPT) |
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